Visualization of 3-D phase structure in confocal and conventional microscopy

Carol J. Cogswell; Colin J. R. Sheppard

doi:10.1117/12.44306

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1 July 1991 Visualization of 3-D phase structure in confocal and conventional microscopy

Carol J. Cogswell, Colin J. R. Sheppard

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Proceedings Volume 1450, Biomedical Image Processing II; (1991) https://doi.org/10.1117/12.44306
Event: Electronic Imaging '91, 1991, San Jose, CA, United States

Abstract

A reflection differential interference contrast (DIC) system has been investigated as a means for imaging phase information in confocal microscopy. Interestingly, this method has an advantage over the split-detector method for differential phase contrast (and its corresponding confocal derivative) in that the background signal decays as the specimen surface is displaced away from the focal plane. Therefore, a series of DIC images from successive planes of focus can be used to produce easily visualized three-dimensional reconstructions.

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Carol J. Cogswell and Colin J. R. Sheppard "Visualization of 3-D phase structure in confocal and conventional microscopy", Proc. SPIE 1450, Biomedical Image Processing II, (1 July 1991); https://doi.org/10.1117/12.44306

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