Optical sensors for process monitoring in biotechnology

F. Ploetz; Carsten Schelp; K. Anders; F. Eberhardt; Thomas-Helmut Scheper; F. Bueckmann

doi:10.1117/12.47142

1 September 1991 Optical sensors for process monitoring in biotechnology

F. Ploetz, Carsten Schelp, K. Anders, F. Eberhardt, Thomas-Helmut Scheper, F. Bueckmann

Proceedings Volume 1510, Chemical and Medical Sensors; (1991) https://doi.org/10.1117/12.47142
Event: ECO4 (The Hague '91), 1991, The Hague, Netherlands

Abstract

The development and application of various optical sensors will be presented. Among these are optical sensors (optrodes) with immobilized enzymes, coenzymes, and labeled antibodies. The NADH formation of coenzyme dependent enzymes was used for detection of lactate, pyrovate mannitol, ethanol, and formate. An enzyme optrode based on a pH-optrode as a transducer for the monitoring of urea and penicillin in fermentation media was developed. For preparing an oxygen optrode, oxygen-sensitive fluorophores were entrapped in a gaspermeable silicone matrix that is attached to the distal end of a bifurcated fiber optic waveguide bundle. By labeling of immuncomponent with fluorophores or enzymes, which transpose fluorophores or chromophores, immunreactions were observed by an optical sensors.

Citation Download Citation

F. Ploetz, Carsten Schelp, K. Anders, F. Eberhardt, Thomas-Helmut Scheper, and F. Bueckmann "Optical sensors for process monitoring in biotechnology", Proc. SPIE 1510, Chemical and Medical Sensors, (1 September 1991); https://doi.org/10.1117/12.47142

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