1 November 1991 Imaging of tumors by time-delayed laser-induced fluorescence
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Abstract
A technique to improve signal-to-background ratio in fluorescence images of superficially growing tumors marked with photosensitizers is described. Time-delayed detection of fluorescence following pulsed-laser excitation allows suppression of the autofluorescence background falling into the fluorescence band of the photosensitizer. This technique exploits the longer fluorescence-decay times of porphyrin-based photosensitizers compared to average decay times of tissue autofluorescence. The feasibility of time-delayed fluorescence imaging of tumors has been demonstrated in vitro. From time-delayed fluorescence spectra the authors infer that the ratio between photosensitizer signal and autofluorescence background can be improved by about one order of magnitude.
© (1991) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
M. Kohl, Joerg Neukammer, Uwe Sukowski, Herbert H. Rinneberg, Hanns-joerg Sinn, E. A. Friedrich, Georgi Graschew, Peter M. Schlag, Dieter Woehrle, "Imaging of tumors by time-delayed laser-induced fluorescence", Proc. SPIE 1525, Future Trends in Biomedical Applications of Lasers, (1 November 1991); doi: 10.1117/12.48208; https://doi.org/10.1117/12.48208
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