New spectrally resolved confocal scanning laser microscope

John W. Bowron; Savvas Damaskinos; Arthur E. Dixon

doi:10.1117/12.134894

1 February 1992 New spectrally resolved confocal scanning laser microscope

John W. Bowron, Savvas Damaskinos, Arthur E. Dixon

Proceedings Volume 1556, Scanning Microscopy Instrumentation; (1992) https://doi.org/10.1117/12.134894
Event: San Diego, '91, 1991, San Diego, CA, United States

Abstract

We have developed a spectrally resolved confocal microscope with high photon efficiency for photoluminescence and fluorescence measurements. A scanning grating is placed inside the detection arm of the microscope so that the diffraction-limited spot on the sample acts like the entrance slit, and the detector pinhole acts like the exit slit of a standard monochromator. The pinhole also performs the same function as the detector pinhole in a standard confocal microscope. This arrangement has better photon efficiency than focusing the light from the detector pinhole of a standard confocal scanning laser microscope onto the entrance slit of a grating monochromator. This configuration also produces higher spectral resolution and is more flexible than one in which bandpass filters are placed in the detection arm. The microscope is described and measurements of the spectral and axial resolutions are presented. Axial resolution measurements were made using a planar sample that is both reflecting and photoluminescent. Spectrally resolved photoluminescence and fluorescence images are presented.

Citation Download Citation

John W. Bowron, Savvas Damaskinos, and Arthur E. Dixon "New spectrally resolved confocal scanning laser microscope", Proc. SPIE 1556, Scanning Microscopy Instrumentation, (1 February 1992); https://doi.org/10.1117/12.134894

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