1 March 1992 Theoretical considerations for evanescent-wave immunosensors in biomedical applications
Author Affiliations +
Using evanescent wave immunosensors, a fluorescent labeled analyte may be concentrated within the active surface region by a combination of diffusion to the fiber surface and trapping of the molecule or complex by an antigen-antibody affinity reaction. With the dye B- phycoerythrin, approximately 1.5 X 10-22 moles may be sensed over a 1 mm2 surface. From this number (as determined for a particular dye and instrumentation system) and antibody affinity constants, limits to assay sensitivity can be calculated and the kinetics modeled.
© (1992) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Donna S. Orvedahl, Donna S. Orvedahl, Walter F. Love, Walter F. Love, Rudolf E. Slovacek, Rudolf E. Slovacek, } "Theoretical considerations for evanescent-wave immunosensors in biomedical applications", Proc. SPIE 1587, Chemical, Biochemical, and Environmental Fiber Sensors III, (1 March 1992); doi: 10.1117/12.56545; https://doi.org/10.1117/12.56545

Back to Top