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5 March 1993 Photodynamic effect of chlorophyll derivative with pulsed copper-vapor laser on human rectal carcinoma in vitro
Li-Song Teng; Shu Zheng; Jin-Min Wu; Hai Yu; Zhao-Min Cheng
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Proceedings Volume 1616, International Conference on Photodynamic Therapy and Laser Medicine; (1993) https://doi.org/10.1117/12.137029
Event: International Conference on Photodynamic Therapy and Laser Medicine, 1991, Beijing, China
Abstract
In the present study, the effects of a newly developed photosensitizer chrolophyll derivative (CPD4) from Chinese Medicine Cansha (silkworm excrement) with pulsed copper vapor laser and pumped-dye laser (510, 578, 630, and 670 nm wavelength) on human rectal cancer cell line HR8348 was investigated. Hematoporphyrin derivative (YHPD) was used as a positive control. The relationship between CPD4 dose, the laser wavelength, and laser energy was explored. The MTT rapid colorimetric assay was used as a measuring indicator of photodynamic cytotoxicity and compared with 3H-thymidine incorporation assay and colony-forming assay. The photodynamic sensitivity of different cell lines was observed. The results showed that CPD4-induced HR8348 cells irradiated with pulsed copper vapor laser displayed a markedly photodynamic effect.
© (1993) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
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Li-Song Teng, Shu Zheng, Jin-Min Wu, Hai Yu, and Zhao-Min Cheng "Photodynamic effect of chlorophyll derivative with pulsed copper-vapor laser on human rectal carcinoma in vitro", Proc. SPIE 1616, International Conference on Photodynamic Therapy and Laser Medicine, (5 March 1993); https://doi.org/10.1117/12.137029
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