Toxicity, pharmacokinetics, and photodynamic properties of chlorin e6

Gennady Kostenich; Ivan N. Zhuravkin; G. P. Gurinovich; Edvard A. Zhavrid M.D.

doi:10.1117/12.136990

5 March 1993 Toxicity, pharmacokinetics, and photodynamic properties of chlorin e6

Gennady Kostenich, Ivan N. Zhuravkin, G. P. Gurinovich, Edvard A. Zhavrid M.D.

Author Affiliations +

Proceedings Volume 1616, International Conference on Photodynamic Therapy and Laser Medicine; (1993) https://doi.org/10.1117/12.136990
Event: International Conference on Photodynamic Therapy and Laser Medicine, 1991, Beijing, China

Abstract

Toxicity, pharmacokinetics, and the tumor damage effect of chlorin e₆ after light irradiation were studied. The results show that chlorin e₆ LD50 value in C57Bl mice was 189 +/- 10 mg/kg, in non-inbred white rats it was 99 +/- 14 mg/kg 14 days after the agent iv injection. The concentration of chlorin e₆ in blood, liver, kidney, spleen, and tumors (sarcoma M-1 and sarcoma 45) of the rats was determined by the fluorescence method 3, 6, 12, 18, 24, 48, and 72 hours after the agent iv injection at the dose of 10 mg/kg. For this purpose chlorin e₆ was extracted from tissues by detergent triton X-100. The depth of necrosis spreading in tumor tissue was evaluated after chlorin e₆ injection at the doses of 1 - 10 mg/kg and subsequent irradiation by a krypton laser with light energy density of 90 J/cm², using the method of vital staining with Evans blue. It was found that depending on the agent dose and time interval between chlorin e₆ injection and photoradiation, the depth of tumor necrosis varied from 4.0 to 16.6 mm in sarcoma M-1 and from 5.0 to 15.0 in sarcoma 45.

Citation Download Citation

Gennady Kostenich, Ivan N. Zhuravkin, G. P. Gurinovich, and Edvard A. Zhavrid M.D. "Toxicity, pharmacokinetics, and photodynamic properties of chlorin e6", Proc. SPIE 1616, International Conference on Photodynamic Therapy and Laser Medicine, (5 March 1993); https://doi.org/10.1117/12.136990

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