Lifetime-resolved fluorescence-detected circular dichroism: a new technique for probing macromolecular structures

Linda B. McGown; Karen Wu; Lei Geng

doi:10.1117/12.58233

1 April 1992 Lifetime-resolved fluorescence-detected circular dichroism: a new technique for probing macromolecular structures

Linda B. McGown, Karen Wu, Lei Geng

Author Affiliations +

Proceedings Volume 1640, Time-Resolved Laser Spectroscopy in Biochemistry III; (1992) https://doi.org/10.1117/12.58233
Event: OE/LASE '92, 1992, Los Angeles, CA, United States

Abstract

Theory and instrumentation of lifetime-resolved fluorescence-detected circular dichroism spectroscopy (LRFDCD) are described. Advantages of LRFDCD over the individual techniques of fluorescence lifetime determinations and circular dichroism spectroscopy are discussed. LRFDCD is implemented on a frequency-domain fluorescence lifetime instrument that is modified for fluorescence-detected circular dichroism measurements.

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Linda B. McGown, Karen Wu, and Lei Geng "Lifetime-resolved fluorescence-detected circular dichroism: a new technique for probing macromolecular structures", Proc. SPIE 1640, Time-Resolved Laser Spectroscopy in Biochemistry III, (1 April 1992); https://doi.org/10.1117/12.58233

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