Translator Disclaimer
1 April 1992 New method for the simultaneous measurement of fluorescence and phosphorescence depolarizations
Author Affiliations +
Abstract
A new technique for the simultaneous measurement of fluorescence and phosphorescence depolarization is reported. The technique is based on a newly developed photomultiplier gain suppression technique. The gain suppression characteristics of the circuit were studied by applying a constant level of incident light. To demonstrate this new technique, a solid sample of the triplet probe eosin was excited by a pulsed, frequency doubled Nd:YAG laser; the resulting orthogonally-polarized emissions were collected by two balanced photomultiplier (PMTs). The gains of the PMTs were suppressed for the duration of the fluorescence emission, and subsequently returned to normal, in order to keep the measured fluorescence and phosphorescence signals at the same level. The suppression of the gain was controlled by using a specially designed dual-channel PMT gain suppression circuit. The signals from the PMTs were recorded by two digital oscilloscopes, one set at a fast sweep time in order to measure the transient fluorescence depolarization and the other set at a slow sweep time in order to measure the slow phosphorescence depolarization. The anisotropies can then be deduced from the two sets of recordings.
© (1992) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Liqun Yang, Daniel McStay, Abhay Sharma, Peter J. Quinn, and Alan J. Rogers "New method for the simultaneous measurement of fluorescence and phosphorescence depolarizations", Proc. SPIE 1640, Time-Resolved Laser Spectroscopy in Biochemistry III, (1 April 1992); https://doi.org/10.1117/12.58220
PROCEEDINGS
7 PAGES


SHARE
Advertisement
Advertisement
Back to Top