1 April 1992 Spark source infrared fluorometry
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Abstract
The application of our development of 750 nm excitation from an argon-hydrogen filled spark source to time-resolved fluorescence probe studies of lipid membranes and inverse micelles is reported. The laser dye IR-140 was studied using the single-photon counting technique both in a lipid membrane of L-(alpha) dipalmitoylphosphatidylcholine (DPPC) and in sodium sulfosuccinic acid bis (2-ethylhexyl) ester (AOT) in iso-octane. In DPPC a dramatic change in the fluorescence behavior of IR-140 is observed between the gel and liquid crystalline phases. In inverse micelles of AOT an increase in the intensity of the peak fluorescence emission and a decrease in fluorescence lifetime is noticed on increasing the water content.
© (1992) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Graham Hungerford, Graham Hungerford, David J. S. Birch, David J. S. Birch, Robert E. Imhof, Robert E. Imhof, } "Spark source infrared fluorometry", Proc. SPIE 1640, Time-Resolved Laser Spectroscopy in Biochemistry III, (1 April 1992); doi: 10.1117/12.58218; https://doi.org/10.1117/12.58218
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