1 April 1992 Time-resolved ratio method for multicomponent fluorescence pattern analysis using a fluorescence lifetime imaging system
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Proceedings Volume 1640, Time-Resolved Laser Spectroscopy in Biochemistry III; (1992); doi: 10.1117/12.58235
Event: OE/LASE '92, 1992, Los Angeles, CA, United States
Abstract
Time-resolved ratio method is described for multicomponent pattern analysis by using a fluorescence lifetime imaging system and a ratio method calculation. Nanosecond-level time- resolved fluorescence images of a sample under a pulsed light excitation were detected directly by using a gated microchannel plate (MCP) image intensifier coupled to a CCD camera. Differences in fluorescence lifetimes of components in which each component has similar fluorescence wavelength characteristics were positively utilized to estimate every component spatial distribution. Spatial deconvolution of multicomponent fluorescence data was rapidly carried out from a set of time-resolved, two-dimensional fluorescence data. An application of a multigate time-resolved ratio method for fluorescence pattern analysis was provided. From the results, it is shown that the new method can be successfully used for multicomponent fluorescence pattern analysis at a reasonable signal-to-noise ratio.
© (1992) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Xue Feng Wang, Takeshi Uchida, Shigeo Minami, "Time-resolved ratio method for multicomponent fluorescence pattern analysis using a fluorescence lifetime imaging system", Proc. SPIE 1640, Time-Resolved Laser Spectroscopy in Biochemistry III, (1 April 1992); doi: 10.1117/12.58235; https://doi.org/10.1117/12.58235
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KEYWORDS
Luminescence

Image intensifiers

Time resolved spectroscopy

Fluorescence lifetime imaging

Imaging systems

CCD cameras

Microchannel plates

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