1 June 1992 Laser zona dissection using short-pulse ultraviolet lasers
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Proceedings Volume 1650, Medical Lasers and Systems; (1992) https://doi.org/10.1117/12.137499
Event: OE/LASE '92, 1992, Los Angeles, CA, United States
The interaction of pulsed ultraviolet radiation with the zona pellucida of human oocytes which had failed to fertilize in standard IVF cycles, was investigated. Two lasers were studied: a 100 ps pulsed Nd:YAG with a nonlinear crystal emitting light at 266 nm, and a 15 ns XeCl excimer laser with 308 nm radiation. Incisions in the zona were made by aiming the beam tangentially to the oocyte. The results indicate superior, high precision performance by the excimer laser creating trenches as narrow as 1 micrometers and as shallow as 1 micrometers . The incision size was found to be sensitive to the laser's energy and to the position of the microscope's objective focal plane, but relatively insensitive to the laser pulse repetition rate. Once the minimum spot size was defined by the system parameters, the laser beam was used to curve out any desired zona shape. This laser microsurgery technique as applied to partial zone dissection or zona drilling could prove very useful as a high-precision, non-contact method for treatments of low fertilization rate and for enhancing embryo implantation rates in patients undergoing IVF treatments.
© (1992) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Joseph Neev, Joseph Neev, Yona Tadir, Yona Tadir, Peter D. Ho, Peter D. Ho, William E. Whalen, William E. Whalen, Richardo H. Asch, Richardo H. Asch, Teri Ord, Teri Ord, Michael W. Berns, Michael W. Berns, } "Laser zona dissection using short-pulse ultraviolet lasers", Proc. SPIE 1650, Medical Lasers and Systems, (1 June 1992); doi: 10.1117/12.137499; https://doi.org/10.1117/12.137499

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