19 May 1992 Nanometer-size metal particle analysis in microscopic biological images
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In order to automatically analyze electron immunomicroscopy images, we have developed a computerized scheme for the detection and characterization of nanometer-size metal particles. This scheme consists of a preprocessing step aimed at enhancing image features and of an extraction step which performs the analysis of the particles. The method is designed to work irrespective of background and scene illumination. Results are presented for the analysis of immunogold labelling of muscle tissues.
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Jean-Christophe Olivo-Marin, "Nanometer-size metal particle analysis in microscopic biological images", Proc. SPIE 1657, Image Processing Algorithms and Techniques III, (19 May 1992); doi: 10.1117/12.58359; https://doi.org/10.1117/12.58359

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