26 June 1992 Signal strength and its effects on processing of confocal images
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Abstract
The effects of detector size and shape in the imaging properties of confocal microscopes are discussed. The presence of stray light in the optical system, shot noise on the beam and detector performance limit the signal to noise ratio available. The effects on the noise performance of the extended-focus (mean) and autofocus (peak) algorithms for forming image projections are presented. Consideration of these various parameters allows the microscope user to obtain the best performance from his instrument for particular applications.
© (1992) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Colin J. R. Sheppard, Colin J. R. Sheppard, Min Gu, Min Gu, Maitreyee Roy, Maitreyee Roy, } "Signal strength and its effects on processing of confocal images", Proc. SPIE 1660, Biomedical Image Processing and Three-Dimensional Microscopy, (26 June 1992); doi: 10.1117/12.59557; https://doi.org/10.1117/12.59557
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