CONFERENCE PROCEEDINGS
Advanced Search >
Home > Proceedings > Volume 1660 > Article
Translator Disclaimer
26 June 1992 Stage-scanned chromatically aberrant confocal microscope for 3-D surface imaging
Mark Anthony Browne; Olusola Akinyemi; Francis Crossley; Duncan T. B. Stacey
Author Affiliations +
Proceedings Volume 1660, Biomedical Image Processing and Three-Dimensional Microscopy; (1992) https://doi.org/10.1117/12.59583
Event: SPIE/IS&T 1992 Symposium on Electronic Imaging: Science and Technology, 1992, San Jose, CA, United States
Abstract
A method for full-field surface profiling in the tandem scanning confocal microscope has been previously described. The technique utilizes chromatic aberration to produce an extended and color coded focal volume. Planes at different axial depths within this volume correspond to the foci of different wavelengths through the intentionally aberrant system. To determine the height of any point within the field, the confocal detection system must be capable of identifying the wavelength of the most intensely reflected light from that point. Hence the relative sensitivity of the detector and the spectral response of the imaging system are important parameters. We have utilized xenon and mercury arc sources and compared results with various types of objective lens. One potential limitation of this microscope is the fact that the introduction of longitudinal chromatic aberration affects the correction of the optics for plan imaging, i.e., introduces spherical aberrations, but the technique is rapid and produces results well correlated with more conventional techniques. To allow detailed study of the use of a chromatic 3-D probe for surface imaging we have designed and constructed a stage- scanned instrument which uses on-axis optics and is therefore free from spherical aberrations and can be corrected optimally. The instrument is described and results presented for single pass 3-D imaging.
© (1992) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation Download Citation
Mark Anthony Browne, Olusola Akinyemi, Francis Crossley, and Duncan T. B. Stacey "Stage-scanned chromatically aberrant confocal microscope for 3-D surface imaging", Proc. SPIE 1660, Biomedical Image Processing and Three-Dimensional Microscopy, (26 June 1992); https://doi.org/10.1117/12.59583
PROCEEDINGS
 10 PAGES
DOWNLOAD PDF SAVE TO MY LIBRARY
SHARE
GET CITATION
< Previous Article
|
Next Article >
Advertisement
Advertisement
KEYWORDS
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top