Three-dimensional representation of biological objects as reconstructed from sequential image planes, Part I: the problem of image realignment

Tomas Jansson; Martin Rydmark; Tomas Gustavsson; Claes-Henric Berthold; Ragnar Pascher; Thomas Skoglund

doi:10.1117/12.59546

26 June 1992 Three-dimensional representation of biological objects as reconstructed from sequential image planes: Part I - the problem of image realignment

Tomas Jansson, Martin Rydmark, Tomas Gustavsson, Claes-Henric Berthold, Ragnar Pascher, Thomas Skoglund

Author Affiliations +

Proceedings Volume 1660, Biomedical Image Processing and Three-Dimensional Microscopy; (1992) https://doi.org/10.1117/12.59546
Event: SPIE/IS&T 1992 Symposium on Electronic Imaging: Science and Technology, 1992, San Jose, CA, United States

Abstract

We describe a method for automatic realignment of consecutive 2-D microscopic images of brain cortex. The procedure is capable of carrying out high-quality realignment of 10 - 20 images per hour. The resulting image stack can be viewed in real-time by cinematographic animation or used for 3-D object reconstruction. The technique does not rely on expensive hardware, but can be implemented on low-cost PCs and workstations.

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Tomas Jansson, Martin Rydmark, Tomas Gustavsson, Claes-Henric Berthold, Ragnar Pascher, and Thomas Skoglund "Three-dimensional representation of biological objects as reconstructed from sequential image planes: Part I - the problem of image realignment", Proc. SPIE 1660, Biomedical Image Processing and Three-Dimensional Microscopy, (26 June 1992); https://doi.org/10.1117/12.59546

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