24 September 1993 Picosecond 3D fluorescence and absorption spectroscopy of a manipulated microparticle
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Proceedings Volume 1711, High-Performance Optical Spectrometry; (1993) https://doi.org/10.1117/12.155641
Event: High Performance Optical Spectrometry, 1992, Warsaw, Poland
Abstract
Dynamic microspectroscopy systems have been developed for elucidating photophysical and photochemical processes occuring in small volumes. The first system concerns fluorescence spectroscopic measurements based on a timecorrelated single photon counting and a confocal fluorescence microscope, which makes it possible to observe the fluorescence dynamics with both picosecond time-resolution and micrometer three-dimensional space-resolution. The second system is for absorption spectroscopic measurements. Picosecond pump and probe beams are coaxially and confocally introduced into a microscope, giving the depth profile in addition to lateral information. These systems are further combined with a trapping laser which optically tweezers a single microparticle against its Brownian motion. Laser trapping thus introduces the precise time- and space-resolved measurement of particles. To analyze rise and decay curves as a function of position and wavelength, a new fast and accurate analyzing method using a convolved autoregressive model has been proposed. The computation time of this method is much shorter than that of the conventional methods. The present systems have been applied to various kinds of microparticles such as microcapsules, polymer latexes, and oil droplets.
© (1993) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Keiji Sasaki, "Picosecond 3D fluorescence and absorption spectroscopy of a manipulated microparticle", Proc. SPIE 1711, High-Performance Optical Spectrometry, (24 September 1993); doi: 10.1117/12.155641; https://doi.org/10.1117/12.155641
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