9 March 1993 Substrate effect on the enhanced biodegradation of carbon tetrachloride
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The effects of different substrates on the biotransformation of heavily chlorinated hydrocarbons under anaerobic conditions were investigated to evaluate the feasibility of in-situ bioremediation of the contaminated groundwaters by amending different substrates. The substrate-fed batches were anaerobically incubated with the addition of either acetate, glucose, methanol, or dissolved organic matter (DOM) with concentrations ranging from 10 to 30 mg/L. Experimental results demonstrated that the effect of the substrates on the dechlorination of the compounds varied. The removal efficiency was observed greatest for glucose- then methanol- and acetate-fed batches and least for DOM-fed batches. The sequence of the enhancement efficiency is consistent with the sequence of the reducing potentials of these substrates. Changing the substrate concentration could vary the dechlorination capability of the system. The viable counts of microorganisms determined by the direct epifluorescence counting technique showed that the batches with higher concentration of the supplemental substrate produced higher bacterial cell numbers. Moreover, from microscopic observations, different compositions of bacterial population were found. Small-sized bacteria with spheric shape were observed when culture bottles were amended with either acetate or DOM, whereas large-sized bacteria with rod-shape were predominant for bottles amended with glucose. Also, higher DNA contents were demonstrated for glucose-fed batches.
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Shian Chee Wu, Shian Chee Wu, Rueyan Doong, Rueyan Doong, } "Substrate effect on the enhanced biodegradation of carbon tetrachloride", Proc. SPIE 1716, International Conference on Monitoring of Toxic Chemicals and Biomarkers, (9 March 1993); doi: 10.1117/12.140264; https://doi.org/10.1117/12.140264

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