13 January 1993 Effects of soft x-ray irradiation on cell ultrastructure
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Abstract
The future of x-ray microscopy lies mainly in its potential for imaging fresh, hydrated biological material at a resolution superior to that of light microscopy. For the image to be accepted as representing the cellular organization of the living cell, it is essential that artefacts are not introduced as a result of the image collection system. One possible source of artefacts is cellular damage resulting form the irradiation of the material with soft x rays. Cells of the unicellular alga Chlorella have been examined by transmission electron microscopy (TEM) following exposure to different doses of monochromatic (380 eV) soft x rays. Extreme ultrastructural damage has been detected following doses of 103 - 104 Gy, in particular loss of cellular membranes such as the internal thylakoid membranes of the chloroplast. This is discussed in relation to dosage commonly used for imaging by soft x-ray microscopy.
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Thomas W. Ford, Thomas W. Ford, Anton M. Page, Anton M. Page, Guy F. Foster, Guy F. Foster, Anthony D. Stead, Anthony D. Stead, } "Effects of soft x-ray irradiation on cell ultrastructure", Proc. SPIE 1741, Soft X-Ray Microscopy, (13 January 1993); doi: 10.1117/12.138748; https://doi.org/10.1117/12.138748
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