Flash contact x-ray microscopy of biological specimen in water

Toshihisa Tomie; Hazime Shimizu; Toshikazu Majima; Toshihiko Kanayama; Mitsuo Yamada; Eisuke Miura

doi:10.1117/12.138725

13 January 1993 Flash contact x-ray microscopy of biological specimen in water

Toshihisa Tomie, Hazime Shimizu, Toshikazu Majima, Toshihiko Kanayama, Mitsuo Yamada, Eisuke Miura

Proceedings Volume 1741, Soft X-Ray Microscopy; (1993) https://doi.org/10.1117/12.138725
Event: San Diego '92, 1992, San Diego, CA, United States

Abstract

From the shot noise consideration in x-ray microscopy it is shown that, for 50 nm resolution, a few tens mJ/cm² energy of 3 nm x ray is required on the specimen, and required x ray energy density increases with the fourth power of the resolving power. X-ray images of biological specimens in water taken by flash contact microscopy with a laser-plasma source are presented to discuss the prospects of x-ray microscopy for biological applications. Forty nanometer diameter biological components are observed in an x-ray image. The attempt of observing thick specimens by short wavelength x rays produced by a 10 ps duration laser pulse is also reported. Technical issues for further improving contact microscopy, including the feasibility of stereo-microscopy, are discussed.

Citation Download Citation

Toshihisa Tomie, Hazime Shimizu, Toshikazu Majima, Toshihiko Kanayama, Mitsuo Yamada, and Eisuke Miura "Flash contact x-ray microscopy of biological specimen in water", Proc. SPIE 1741, Soft X-Ray Microscopy, (13 January 1993); https://doi.org/10.1117/12.138725

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