13 January 1993 High-resolution x-ray stereomicroscopy: true three-dimensional imaging of biological samples
Author Affiliations +
X-ray microscopy has the potential to become a powerful tool for the study of biological samples, allowing the imaging of intact cells and subcellular organelles in an aqueous environment at resolutions previously achievable only by electron microscopy. The ability to examine a relatively thick sample raises the issue of superposition of objects from multiple planes within the sample, making difficult the interpretation of conventional, orthogonally projected images. This paper describes our early attempts at developing three-dimensional methods for x-ray microimaging: the first to use x-ray optics, and to our knowledge, the first demonstrating sub-visible resolutions and natural contrast. These studies were performed using the scanning transmission x-ray microscope (STXM) at the National Synchrotron Light Source, Brookhaven National Laboratory.
© (1993) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Billy W. Loo, Billy W. Loo, Shawn P. Williams, Shawn P. Williams, W. Lin, W. Lin, Willaim H. Love, Willaim H. Love, Stanley Meizel, Stanley Meizel, Stephen S. Rothman, Stephen S. Rothman, "High-resolution x-ray stereomicroscopy: true three-dimensional imaging of biological samples", Proc. SPIE 1741, Soft X-Ray Microscopy, (13 January 1993); doi: 10.1117/12.138755; https://doi.org/10.1117/12.138755


Back to Top