Fluorescence spectroscopy has long proven to be a valuable tool in fiber optic applications. A large number of publications have addressed different fiber optic applications using fluorescent probe molecules. Since most probe molecules absorb in the UV/Vis part of the electromagnetic spectrum, the majority of these applications address the use of visible fluorophores. However, the utilization of the longer wavelength part of the spectrum may be advantageous due to its relatively low interference. Biological applications of this longer wavelength spectral region may be especially advantageous if semiconductor lasers are used as light sources. Laser diodes have all the properties of other types of lasers with the added benefits of compactness and low price. To utilize these advantages, however, new NIR absorbing probe molecules need to be developed. Certain requirements, e.g., chemical stability, presence of functional groups for binding to the fiber, etc., need to be met for using these NIR chromophores in fiber optic applications. These NIR fluorophores may be incorporated into a fiber optic probe and used for determining analytically important properties. In this paper examples of the use of NIR fluorophores are given.