30 April 1993 Spectral interferometric immunoassay with synthetic lipid-anchored polypeptide antigens
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Spectral interferometric investigations, which were carried out at thin dielectric layers with a novel fixation procedure for synthetic antigens, are reported. This work is aimed at an optical, regenerable, label-free immunosensor for antibody determinations. Most optical biosensor approaches require fixation of some kind of receptor to the sensor surface. Simple adsorption leads to washout effects and often prohibits sensor regeneration. Covalent fixation usually decreases receptor affinity significantly. In this paper we report a noncovalent fixation procedure, based on synthetic, lipid anchored peptide antigens. Fixation is achieved by strong hydrophobic interaction between the pretreated sensor surface and a lipid anchor covalently linked to the antigen. Spectral interferometry uses short coherent (white) light interference for the determination of surface and volume effects at thin films. This method has been successfully applied to hydrocarbon sensing and solid phase adsorption immunoassay.
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Andreas Brecht, Andreas Brecht, Guenter Gauglitz, Guenter Gauglitz, Werner Beck, Werner Beck, Renate Spohn, Renate Spohn, Guenter Jung, Guenter Jung, } "Spectral interferometric immunoassay with synthetic lipid-anchored polypeptide antigens", Proc. SPIE 1796, Chemical, Biochemical, and Environmental Fiber Sensors IV, (30 April 1993); doi: 10.1117/12.143567; https://doi.org/10.1117/12.143567

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