30 April 1993 Submicron optical sources for single macromolecule detection
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Nanobit holographic techniques, such as high-energy electron beam lithography, allow apertures of < 50 nm diam. to be fabricated in the metal coating of SPR sensor devices. When the SPR slab waveguide is edge illuminated with a laser, the apertures act as intense scattering centers of the underlying radiation, the scattered light from which is readily detectable by conventional optical microscopy. Given the dimensions of these sub-wavelength optical sources are within an order of magnitude of those of individual biological macromolecules, it should ultimately prove possible to detect interactions between substrate/analyte and individual (labelled) biological macromolecules immobilized in the apertures. The possibility of analyzing the dynamic behavior of single biomacromolecules operating in their natural environment is discussed.
© (1993) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Robert J. G. Carr, Robert J. G. Carr, Sumaia Al-Shukri, Sumaia Al-Shukri, Elaine A. Perkins, Elaine A. Perkins, Thierry Rodard, Thierry Rodard, Tony Atkinson, Tony Atkinson, } "Submicron optical sources for single macromolecule detection", Proc. SPIE 1796, Chemical, Biochemical, and Environmental Fiber Sensors IV, (30 April 1993); doi: 10.1117/12.143509; https://doi.org/10.1117/12.143509

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