Scanning force microscopy and STM imaging of B-chitin

Manfred H. Jericho; P. J. Mulhern; Wenbo Xu; B. L. Blackford; Lawrence Fritz

doi:10.1117/12.146379

4 June 1993 Scanning force microscopy and STM imaging of β-chitin

Manfred H. Jericho, P. J. Mulhern, Wenbo Xu, B. L. Blackford, Lawrence Fritz

Proceedings Volume 1855, Scanning Probe Microscopies II; (1993) https://doi.org/10.1117/12.146379
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

A combination of SEM, STM, and SFM microscopy determined structural and elastic properties of (beta) -chitin, a linear polysaccharide that serves as a skeletal material in some marine species. Each microscopy technique produced artifacts that may lead to incorrect conclusions, but consistent results from multiple techniques improve the confidence in the interpretation. Our studies confirmed the existence of 40 nm X 20 - 50 nm substructures in chitin fibers from the diatom Thalassiosira fluviatilis, and found several surface structures not previously reported. We also investigated a technique to enhance subtle features in images by vibrating an SFM sample stage. The SFM directly measured the Young's Modulus of the fibers to be 1 +/- 0.5 X 10¹¹ N/m².

Citation Download Citation

Manfred H. Jericho, P. J. Mulhern, Wenbo Xu, B. L. Blackford, and Lawrence Fritz "Scanning force microscopy and STM imaging of β-chitin", Proc. SPIE 1855, Scanning Probe Microscopies II, (4 June 1993); https://doi.org/10.1117/12.146379

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