24 June 1993 New advances in 3D visualization of the cornea
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Proceedings Volume 1877, Ophthalmic Technologies III; (1993) https://doi.org/10.1117/12.147516
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States
This paper demonstrates the three-dimensional visualization of the ex vivo rabbit cornea. The cornea is an ideal test specimen to evaluate techniques for 3-D reconstruction and visualization of unstained, unfixed, transparent living tissues from a stack of optical sections. The 0.4 mm thick, transparent, specimen has been optically sectioned into 365 sections using a laser scanning confocal microscope with a water immersion objective. Isometric sampling resulted in near-cubic voxels which compensated for the reduced microscopic resolution in the z axis as compared to x and y resolution. This paper demonstrates the first three-dimensional visualization of the posterior stroma and the basal epithelium of the ex vivo rabbit cornea.
© (1993) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Barry R. Masters, Barry R. Masters, } "New advances in 3D visualization of the cornea", Proc. SPIE 1877, Ophthalmic Technologies III, (24 June 1993); doi: 10.1117/12.147516; https://doi.org/10.1117/12.147516

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