This paper demonstrates the three-dimensional visualization of the ex vivo rabbit cornea. The cornea is an ideal test specimen to evaluate techniques for 3-D reconstruction and visualization of unstained, unfixed, transparent living tissues from a stack of optical sections. The 0.4 mm thick, transparent, specimen has been optically sectioned into 365 sections using a laser scanning confocal microscope with a water immersion objective. Isometric sampling resulted in near-cubic voxels which compensated for the reduced microscopic resolution in the z axis as compared to x and y resolution. This paper demonstrates the first three-dimensional visualization of the posterior stroma and the basal epithelium of the ex vivo rabbit cornea.