Intracellular events associated with uptake of a monomeric photosensitizer, benzoporphyrin derivative monoacid ring A (BPD), and its subsequent activation with light

P. Mark Curry; Anna M. Richter; Ashok K. Jain; Julia G. Levy

doi:10.1117/12.146318

18 June 1993 Intracellular events associated with uptake of a monomeric photosensitizer, benzoporphyrin derivative monoacid ring A (BPD), and its subsequent activation with light

P. Mark Curry, Anna M. Richter, Ashok K. Jain, Julia G. Levy

Author Affiliations +

Proceedings Volume 1881, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy II; (1993) https://doi.org/10.1117/12.146318
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

Intracellular events associated with photosensitizers and their subsequent activation with light are as yet poorly understood. Using a model photosensitizer, benzoporphyrin derivative, monoacid ring A (BPD), we have studied cellular uptake and release using both cell lines and normal murine splenocytes. These studies showed that maximum uptake was effected rapidly, peaking between 15 and 30 minutes following exposure to BPD. Absolute quantities taken up varies substantially between cell sources, malignant cell lines and activated normal cells taking up significantly higher levels. Release of BPD from cells occurred equally rapidly when they were removed from BPD-containing medium. Phototoxicity studies indicated that more sensitive cell structures were exposed to the photosensitizer with increased time of incubation. Further experiments were conducted to study protein expression in a murine tumor cell line following BPD treatment. We observed that the oxidative stress associated with photodynamic therapy resulted in the induction of a set of heat shock or stress proteins, and that the pattern of expression was similar when tumor cells were treated in vitro and in vivo.

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P. Mark Curry, Anna M. Richter, Ashok K. Jain, and Julia G. Levy "Intracellular events associated with uptake of a monomeric photosensitizer, benzoporphyrin derivative monoacid ring A (BPD), and its subsequent activation with light", Proc. SPIE 1881, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy II, (18 June 1993); https://doi.org/10.1117/12.146318

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