The effects of laser light on mineralized tissues can be studied by histological techniques such as SEM, TEM and light microscopy. Beside surface effects, laser induced tissue changes in the subsurface layers are important markers to control the side effects of laser treatment. To study these depth effects in the irradiated area cross sections of the lased bone or teeth are necessary. Mineralized tissues must usually be decalcified before cutting sections for histological analysis. Due to this process many laser induced alterations in mineralized tissues cannot be observed, especially in dental tissues. To avoid these disadvantages sawing and grinding techniques to cut undecalcified sections were developed.