Measurement for optical properties of mitochondria in vitro

Akio Nakajima; Miya Ishihara; Tsunenori Arai; Yuji Morimoto; Makoto Kikuchi; Fumihiko Kannari; Minoru Obara

doi:10.1117/12.148028

14 July 1993 Measurement for optical properties of mitochondria in vitro

Akio Nakajima; Miya Ishihara; Tsunenori Arai; Yuji Morimoto; Makoto Kikuchi; Fumihiko Kannari; Minoru Obara

Author Affiliations +

Proceedings Volume 1883, Low-Energy Laser Effects on Biological Systems; (1993) https://doi.org/10.1117/12.148028
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

The effectiveness of low-intensity light for clinical therapies is well demonstrated, however, the principles of these therapies are not well understood. Our aim of this study is the investigation of optical properties of mitochondria because it seems to be the most important cellular organella in terms of the light-cell interaction. We measured the optical absorption characteristics of an isolated mitochondrial suspension in the range of 300 to 900 nm by means of the transmission methods with an integral sphere. Mitochondrial specific absorption coefficients (ml/g/cm^-1) were carried out by the transmission spectrum. The specific absorption coefficient at the maximum absorption of cytochrome c_(gamma ) peaked about 560 ml/g/cm^-1 at 418 nm. Many papers described that the effect of the low intensity light to the biological tissue would be drastically changed by the irradiation conditions. We could calculate a deposit energy of irradiated light in a mitochondrion from our results. We think our results may be useful to describe the quantitative explanation in energy balance for the interaction between the light and mitochondria.

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Akio Nakajima, Akio Nakajima, Miya Ishihara, Miya Ishihara, Tsunenori Arai, Tsunenori Arai, Yuji Morimoto, Yuji Morimoto, Makoto Kikuchi, Makoto Kikuchi, Fumihiko Kannari, Fumihiko Kannari, Minoru Obara, Minoru Obara, } "Measurement for optical properties of mitochondria in vitro", Proc. SPIE 1883, Low-Energy Laser Effects on Biological Systems, (14 July 1993); doi: 10.1117/12.148028; https://doi.org/10.1117/12.148028

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