21 July 1993 Dynamic light-scattering study of monodisperse collagen in the less-dilute regime
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Proceedings Volume 1884, Static and Dynamic Light Scattering in Medicine and Biology; (1993) https://doi.org/10.1117/12.148372
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States
Abstract
Physicochemical properties of monodisperse type I collagen were studied using the dynamic light scattering and analytical ultra-centrifugation techniques. The measured osmotic second virial coefficient A2 is much smaller than the excluded volume limit calculated from the length L, diameter b and molecular weight M of collagen. The strength kD of the concentration dependence of the mutual diffusivity *DG of collagen is negative in the sign and large in the magnitude. The relationship kD equals 2A2M - v - k(zetz ) does not hold for collagen, while it holds for fd virus and poly((gamma) - benzyl L-glutamate), where v is the partial specific volume, and k(zetz ) the strength of the concentration dependence of the friction coefficient (zetz) . We conclude from these three lines of evidence that water is a poor solvent of collagen and that there must be very weak attractive interactions among collagen filaments which can easily be destroyed by external perturbations.
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Tadakazu Maeda, Tadakazu Maeda, Michiho Takasaki-Ohsita, Michiho Takasaki-Ohsita, Keiko Satoh, Keiko Satoh, Mayumi Kimura, Mayumi Kimura, } "Dynamic light-scattering study of monodisperse collagen in the less-dilute regime", Proc. SPIE 1884, Static and Dynamic Light Scattering in Medicine and Biology, (21 July 1993); doi: 10.1117/12.148372; https://doi.org/10.1117/12.148372
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