How to make a molecular light switch sequence-specific: tethering of an oligonucleotide to a dipyridophenazine complex of ruthenium (II)

Yonchu Jenkins; Jacqueline K. Barton

doi:10.1117/12.144704

18 May 1993 How to make a molecular light switch sequence-specific: tethering of an oligonucleotide to a dipyridophenazine complex of ruthenium (II)

Yonchu Jenkins, Jacqueline K. Barton

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Proceedings Volume 1885, Advances in Fluorescence Sensing Technology; (1993) https://doi.org/10.1117/12.144704
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

Considerable attention has been given recently to the design and development of nonradiative methods of recognizing DNA in a sequence-specific manner. Earlier, we reported that Ru(bpy)₂dppz²⁺(bpy equals 2,2'-bipyridine, dppz equals dipyrido[3,2:a-2',3':c]phenazine) shows no luminescence in aqueous solution, but upon intercalation into double-helical DNA, bright photoluminescence is observed (A. E. Friedman, et al., J. Am. Chem. Soc., 1990, 112, 4960). Based upon this observation, a sequence-specific molecular light switch has been designed in which a dppz complex of ruthenium(II) is tethered onto an oligonucleotide. An oligonucleotide modified at its 5 foot end has been constructed by coupling the sequence 5'-H₂N(CH₂)₆AGTGCCAAGCTTGCA-3' to Ru(phen')₂dppz²⁺ (phen' equals 5-amido-glutaric acid-1,10-phenanthroline). Like the parent complex Ru(bpy)₂dppz²⁺, the single-stranded metal-oligonucleotide conjugate shows little detectable luminescence in aqueous solution. Addition of the complementary strand results in intense photoluminescence; time-resolved studies show that the emission is biphasic with excited state lifetimes of 500 (60%) and 110 (40%) ns. As expected, addition of a non- complementary strand produces no luminescence enhancement over that of the single-stranded metal-oligonucleotide. These results demonstrate that this oligonucleotide derivatized metal complex can be used to recognize and target specific sequences on DNA, a valuable feature which may lead to interesting and novel applications in hybridization technology.

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Yonchu Jenkins and Jacqueline K. Barton "How to make a molecular light switch sequence-specific: tethering of an oligonucleotide to a dipyridophenazine complex of ruthenium (II)", Proc. SPIE 1885, Advances in Fluorescence Sensing Technology, (18 May 1993); https://doi.org/10.1117/12.144704

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