18 May 1993 Ultratrace analysis of biological substances by capillary electrophoresis/semiconductor laser fluorometry
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Proceedings Volume 1885, Advances in Fluorescence Sensing Technology; (1993); doi: 10.1117/12.144729
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States
Abstract
In order to simplify a laser fluorometric detector for capillary electrophoresis a semiconductor laser emitting at 660 nm is used as an exciting source. A labeling reagent fluorescent in the deep-red region is newly synthesized and used for determination of amino acids. The separation resolution is several hundred thousand and the detection limit is subattomole levels. Protein is also labeled by the dye developed and is measured at subfemtomole levels. Contrarily, nonfluorescent samples are detected by indirect fluorometry. Amino acids, flavin adenine dinucleotide (FAD), and deoxyadenosine monophosphate (dAMP) are determined, the detection limit being subpicomole levels.
© (1993) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Tetsuhiro Fuchigami, Totaro Imasaka, Masanobu Shiga, "Ultratrace analysis of biological substances by capillary electrophoresis/semiconductor laser fluorometry", Proc. SPIE 1885, Advances in Fluorescence Sensing Technology, (18 May 1993); doi: 10.1117/12.144729; https://doi.org/10.1117/12.144729
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KEYWORDS
Capillaries

Semiconductor lasers

Luminescence

Biological research

Molecules

Proteins

Molecular biology

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