Laser-induced autofluorescence of cells and tissue

Karsten Koenig; Herbert Schneckenburger; Angelika C. Rueck; Rudolf W. Steiner; Heinrich Walt

doi:10.1117/12.151184

27 August 1993 Laser-induced autofluorescence of cells and tissue

Karsten Koenig, Herbert Schneckenburger, Angelika C. Rueck, Rudolf W. Steiner, Heinrich Walt

Proceedings Volume 1887, Physiological Imaging, Spectroscopy, and Early-Detection Diagnostic Methods; (1993) https://doi.org/10.1117/12.151184
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

The laser induced in-vivo autofluorescence in the visible spectral region of cells and tissues was studied using stationary and time-resolved fluorescence measurements as well as video- intensified- and confocal laser-scanning microscopy. Due to their excitation and emission spectra and their decay kinetics (ps- and ns-region) at least three intrinsic fluorophores were so far differentiated and attributed to the reduced pyridine coenzymes NADH/NADPH, free and bound flavin molecules and endogenous porphyrins. It was found, that defects in the mitochondrial respiratory chain as well as photodynamically or chemically induced cell destruction results in an increase of the fluorescence intensity in the blue/green spectral range.

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Karsten Koenig, Herbert Schneckenburger, Angelika C. Rueck, Rudolf W. Steiner, and Heinrich Walt "Laser-induced autofluorescence of cells and tissue", Proc. SPIE 1887, Physiological Imaging, Spectroscopy, and Early-Detection Diagnostic Methods, (27 August 1993); https://doi.org/10.1117/12.151184

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