27 August 1993 Laser-induced autofluorescence of cells and tissue
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Proceedings Volume 1887, Physiological Imaging, Spectroscopy, and Early-Detection Diagnostic Methods; (1993) https://doi.org/10.1117/12.151184
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States
Abstract
The laser induced in-vivo autofluorescence in the visible spectral region of cells and tissues was studied using stationary and time-resolved fluorescence measurements as well as video- intensified- and confocal laser-scanning microscopy. Due to their excitation and emission spectra and their decay kinetics (ps- and ns-region) at least three intrinsic fluorophores were so far differentiated and attributed to the reduced pyridine coenzymes NADH/NADPH, free and bound flavin molecules and endogenous porphyrins. It was found, that defects in the mitochondrial respiratory chain as well as photodynamically or chemically induced cell destruction results in an increase of the fluorescence intensity in the blue/green spectral range.
© (1993) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Karsten Koenig, Karsten Koenig, Herbert Schneckenburger, Herbert Schneckenburger, Angelika C. Rueck, Angelika C. Rueck, Rudolf W. Steiner, Rudolf W. Steiner, Heinrich Walt, Heinrich Walt, } "Laser-induced autofluorescence of cells and tissue", Proc. SPIE 1887, Physiological Imaging, Spectroscopy, and Early-Detection Diagnostic Methods, (27 August 1993); doi: 10.1117/12.151184; https://doi.org/10.1117/12.151184
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