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The main problem of endometriosis is the detection of microscopic and atypical lesions. The successful destruction of these endometriotic sites depends on their detection. This study aimed to develop a spectrofluorometric method to increase the sensitivity of detection of endometriosis. A surgical-induced endometriosis was performed in ten rabbits. Five weeks later, the fluorescence of these endometriotic lesions was studied after injection of tamoxifen and local application of eosin. This fluorescence was compared with that of healthy broad ligament and that obtained without tamoxifen and without eosin. A spectral analysis showed a specific fluorescence of eosin-tamoxifen association, more intense than autofluorescence and selectively observed within endometriosis.
A. Brogniez,Serge R. Mordon,Jean-Marie Devoisselle,Denis Querleu, andJean Marc Brunetaud
"Study of experimental endometriosis using fluorescence of eosin-tamoxifen association", Proc. SPIE 1887, Physiological Imaging, Spectroscopy, and Early-Detection Diagnostic Methods, (27 August 1993); https://doi.org/10.1117/12.151189
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A. Brogniez, Serge R. Mordon, Jean-Marie Devoisselle, Denis Querleu, Jean Marc Brunetaud, "Study of experimental endometriosis using fluorescence of eosin-tamoxifen association," Proc. SPIE 1887, Physiological Imaging, Spectroscopy, and Early-Detection Diagnostic Methods, (27 August 1993); https://doi.org/10.1117/12.151189