Monitoring protein dynamics with time-resolved CD and MCD spectroscopy

Robert A. Goldbeck; David S. Kliger

doi:10.1117/12.145257

1 May 1993 Monitoring protein dynamics with time-resolved CD and MCD spectroscopy

Robert A. Goldbeck, David S. Kliger

Proceedings Volume 1890, Biomolecular Spectroscopy III; (1993) https://doi.org/10.1117/12.145257
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

Ellipsometric techniques for time-resolved measurements of visible and near-UV CD and MCD have been introduced in the last few years and used to follow the nanosecond dynamics of protein systems containing suitable chromophores. Recent technical advances are described that permit the extension of fast time-resolved CD measurements into the far-UV (190 - 290 nm) spectral region. This development opens the possibility of using CD spectroscopy to monitor secondary structure changes in real time, at physiological temperatures, during processes such as protein folding. Fast CD techniques also permit the measurement of CD and MCD of photoselected protein chromophores before rotational reorientation restores isotropy. Theoretical expression for the CD and MCD of photoselectively oriented absorbers suggest that additional molecular information might be revealed by such measurements.

Citation Download Citation

Robert A. Goldbeck and David S. Kliger "Monitoring protein dynamics with time-resolved CD and MCD spectroscopy", Proc. SPIE 1890, Biomolecular Spectroscopy III, (1 May 1993); https://doi.org/10.1117/12.145257

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available