24 June 1993 Capillary array electrophoresis: an approach to high-speed high-throughput DNA sequencing
Author Affiliations +
Proceedings Volume 1891, Advances in DNA Sequencing Technology; (1993) https://doi.org/10.1117/12.146714
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States
A laser-excited, confocal-fluorescence scanner is used for high-sensitivity, on-column detection of electrophoresis performed using an array of capillaries. The capabilities of this method and apparatus are illustrated by application to high-speed, high-throughput DNA sequencing. Sanger DNA sequencing fragments are separated on an array of capillaries and then distinguished by using a binary coding scheme that employs only two different fluorescently labeled dye primers to identify four sets of fragments. DNA sequencing results are presented using a 25-capillary array. This apparatus has the capability of sequencing DNA at a rate of approximately 25,000 bases/hour.
© (1993) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Xiaohua C. Huang, Xiaohua C. Huang, Mark A. Quesada, Mark A. Quesada, Richard A. Mathies, Richard A. Mathies, "Capillary array electrophoresis: an approach to high-speed high-throughput DNA sequencing", Proc. SPIE 1891, Advances in DNA Sequencing Technology, (24 June 1993); doi: 10.1117/12.146714; https://doi.org/10.1117/12.146714


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