DNA sequencing by single molecule detection of labeled nucleotides sequentially cleaved from a single strand of DNA

Peter M. Goodwin; Jay A. Schecker; Charles W. Wilkerson Jr.; Mark L. Hammond; W. Patrick Ambrose; James H. Jett; John C. Martin; Babetta L. Marrone; Richard A. Keller; Alberto Haces; Po-Jen Shih; John D. Harding

doi:10.1117/12.146712

24 June 1993 DNA sequencing by single molecule detection of labeled nucleotides sequentially cleaved from a single strand of DNA

Peter M. Goodwin, Jay A. Schecker, Charles W. Wilkerson Jr., Mark L. Hammond, W. Patrick Ambrose, James H. Jett, John C. Martin, Babetta L. Marrone, Richard A. Keller, Alberto Haces, Po-Jen Shih, John D. Harding

Author Affiliations +

Proceedings Volume 1891, Advances in DNA Sequencing Technology; (1993) https://doi.org/10.1117/12.146712
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

We are developing a laser-based technique for the rapid sequencing of large DNA fragments (several kb in size) at a rate of 100 to 1000 bases per second. Our approach relies on fluorescent labeling of the bases in a single fragment of DNA, attachment of this labeled DNA fragment to a support, movement of the supported DNA into a flowing sample stream, sequential cleavage of the end nucleotide from the DNA fragment with an exonuclease, and detection of the individual fluorescently labeled bases by laser-induced fluorescence.

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Peter M. Goodwin, Jay A. Schecker, Charles W. Wilkerson Jr., Mark L. Hammond, W. Patrick Ambrose, James H. Jett, John C. Martin, Babetta L. Marrone, Richard A. Keller, Alberto Haces, Po-Jen Shih, and John D. Harding "DNA sequencing by single molecule detection of labeled nucleotides sequentially cleaved from a single strand of DNA", Proc. SPIE 1891, Advances in DNA Sequencing Technology, (24 June 1993); https://doi.org/10.1117/12.146712

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