Sequencing using pulsed field and image reconstruction

Jaan Noolandi; Robert Forsyth; Chantal Turmel; An-Chang Shi

doi:10.1117/12.146702

24 June 1993 Sequencing using pulsed field and image reconstruction

Jaan Noolandi, Robert Forsyth, Chantal Turmel, An-Chang Shi

Proceedings Volume 1891, Advances in DNA Sequencing Technology; (1993) https://doi.org/10.1117/12.146702
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

The use of pulsed fields in a standard manual sequencing set-up results in the separation of over 2000 bases on a single gel. However visual reading of the sequence from a film is not possible for more than about 800 - 900 bases under the best conditions. The use of image reconstruction and analysis techniques allows the reading of the M13 mp18 sequence up to about 1600 - 1700 bases, and individual bands can be identified above 2000 bases.

Citation Download Citation

Jaan Noolandi, Robert Forsyth, Chantal Turmel, and An-Chang Shi "Sequencing using pulsed field and image reconstruction", Proc. SPIE 1891, Advances in DNA Sequencing Technology, (24 June 1993); https://doi.org/10.1117/12.146702

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