XeCl excimer laser-induced autofluorescence spectroscopy for human cerebral tumor diagnosis: preliminary study

Sigrid Avrillier; Frederic Hor; Michel Desgeorges; Dominique Ettori; Jean Robert Sitbon

doi:10.1117/12.154945

17 September 1993 XeCl excimer laser-induced autofluorescence spectroscopy for human cerebral tumor diagnosis: preliminary study

Sigrid Avrillier, Frederic Hor, Michel Desgeorges, Dominique Ettori, Jean Robert Sitbon

Author Affiliations +

Proceedings Volume 1894, Clinical Applications of Modern Imaging Technology; (1993) https://doi.org/10.1117/12.154945
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

Three-hundred-eight nm laser-induced autofluorescence spectra of the normal human brain, astrocytoma grade IV and glioblastoma grade IV specimens, have been recorded in vitro two hours after surgical resection. Typical fluorescence spectra for normal (N) and malignant (M) tissue show 4 maxima at about 352, 362, 383, and 460 nm. These spectra are analyzed in detail. Subtle differences in normalized spectra of N and M tissues appear to be large enough for diagnosis. Several criteria such as maxima and minima absolute intensity and intensity ratios at typical wavelengths are computed and used to classify the tissue. This preliminary study shows that fluorescence spectroscopy with 308 nm UV excitation could be a valid technique for discriminating tumor types. However, it should be noted that these measurements are made in vitro. Living tissues may have different spectral characteristics, therefore future in vivo investigations must be performed.

Citation Download Citation

Sigrid Avrillier, Frederic Hor, Michel Desgeorges, Dominique Ettori, and Jean Robert Sitbon "XeCl excimer laser-induced autofluorescence spectroscopy for human cerebral tumor diagnosis: preliminary study", Proc. SPIE 1894, Clinical Applications of Modern Imaging Technology, (17 September 1993); https://doi.org/10.1117/12.154945

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