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24 June 1993 Automated chemiluminescence immunoassay measurements
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Proceedings Volume 1895, Ultrasensitive Laboratory Diagnostics; (1993)
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States
Chemiluminescence (CL) detection offers potential for high sensitivity immunoassays (CLIAs). Several approaches were attempted to automate CL measurements. Those include the use of photographic film, clear microtitration plates, and magnetic separation. We describe a photon counting detection apparatus that performs (CLIA) measurements. The CL detector moves toward a disposable reaction vessel to create a light-tight seal and then triggers and integrates a CL signal. The capture uses antibody coated polystyrene microparticles. A porous matrix, which is a part of a disposable reaction tray, entraps the microparticle-captured reaction product. The CL signal emanated off the immune complex immobilized by the porous matrix is detected. The detection system is a part of a fully automated immunoassay analyzer. Methods of achieving high sensitivities are discussed.
© (1993) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Omar S. Khalil, G. P. Mattingly, K. Genger, J. Mackowiak, J. Butler, C. Pepe, T. F. Zurek, and N. Abunimeh "Automated chemiluminescence immunoassay measurements", Proc. SPIE 1895, Ultrasensitive Laboratory Diagnostics, (24 June 1993);


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