Automated chemiluminescence immunoassay measurements

Omar S. Khalil; G. P. Mattingly; K. Genger; J. Mackowiak; J. Butler; C. Pepe; T. F. Zurek; N. Abunimeh

doi:10.1117/12.146728

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24 June 1993 Automated chemiluminescence immunoassay measurements

Omar S. Khalil, G. P. Mattingly, K. Genger, J. Mackowiak, J. Butler, C. Pepe, T. F. Zurek, N. Abunimeh

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Proceedings Volume 1895, Ultrasensitive Laboratory Diagnostics; (1993) https://doi.org/10.1117/12.146728
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

Chemiluminescence (CL) detection offers potential for high sensitivity immunoassays (CLIAs). Several approaches were attempted to automate CL measurements. Those include the use of photographic film, clear microtitration plates, and magnetic separation. We describe a photon counting detection apparatus that performs (CLIA) measurements. The CL detector moves toward a disposable reaction vessel to create a light-tight seal and then triggers and integrates a CL signal. The capture uses antibody coated polystyrene microparticles. A porous matrix, which is a part of a disposable reaction tray, entraps the microparticle-captured reaction product. The CL signal emanated off the immune complex immobilized by the porous matrix is detected. The detection system is a part of a fully automated immunoassay analyzer. Methods of achieving high sensitivities are discussed.

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Omar S. Khalil, G. P. Mattingly, K. Genger, J. Mackowiak, J. Butler, C. Pepe, T. F. Zurek, and N. Abunimeh "Automated chemiluminescence immunoassay measurements", Proc. SPIE 1895, Ultrasensitive Laboratory Diagnostics, (24 June 1993); https://doi.org/10.1117/12.146728

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