24 June 1993 Ultrasensitive detection of single molecules in flowing sample streams by laser-induced fluorescence
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Proceedings Volume 1895, Ultrasensitive Laboratory Diagnostics; (1993) https://doi.org/10.1117/12.146731
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States
We report here on the detection and fluorescence lifetime measurement of single Rhodamine- 110 molecules in a flowing, aqueous sample stream. Time-correlated photon counting (TCPC) used in combination with pulsed excitation allows for the detection, in the presence of significant prompt Raman and Rayleigh background, of photon bursts due to single fluorescent molecules passing through a small detection volume (approximately 1 pL). The fluorescence lifetime of a detected molecule is estimated from the decay curve complied from photon arrival times in the burst.
© (1993) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Peter M. Goodwin, Peter M. Goodwin, Charles W. Wilkerson, Charles W. Wilkerson, W. Patrick Ambrose, W. Patrick Ambrose, Richard A. Keller, Richard A. Keller, "Ultrasensitive detection of single molecules in flowing sample streams by laser-induced fluorescence", Proc. SPIE 1895, Ultrasensitive Laboratory Diagnostics, (24 June 1993); doi: 10.1117/12.146731; https://doi.org/10.1117/12.146731

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