17 June 1993 Phthalocyanine interaction with cells: kinetics of the photodynamic damage and intracellular localization
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Proceedings Volume 1921, Laser Spectroscopy of Biomolecules; (1993) https://doi.org/10.1117/12.146130
Event: Laser Spectroscopy of Biomolecules: 4th International Conference on Laser Applications in Life Sciences, 1992, Jyvaskyla, Finland
Abstract
Phthalocyanine interaction with cultured cells was studied by means of flow-cytometry, laser scanning confocal microscopy, fluorescent and transmission pH-microphotometry. Irradiation of Pc-loaded cells caused the following order of events: increase of Pc intracellular fluorescenceyieldsmitochondria damageyieldschanges of cell surface and/or volumeyields-plasma membrane potential disintegrationyields-severe damage of the cell plasma membrane and nuclear envelope. For all these processes it takes a few hours to reach a stationary value. Pc inside the cells is found in granules surrounding the Golgi apparatus and in the peripheral cytoplasmic region, last ones partially coinciding with the acidic cellular compartments. A temporary decrease of cytoplasmic and lysosomal pH is caused by Pc uptake and irradiation. Artificial acidation of the cytoplasm of Pc-loaded cells resulted in the enhancement of the efficiency of the photodynamic inactivation.
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Elena B. Chernyaeva, Jan Greve, Bart G. de Grooth, A. G.J.M. Van Leeuwen, Marina Yu. Poroshina, L. V. Zhorina, "Phthalocyanine interaction with cells: kinetics of the photodynamic damage and intracellular localization", Proc. SPIE 1921, Laser Spectroscopy of Biomolecules, (17 June 1993); doi: 10.1117/12.146130; https://doi.org/10.1117/12.146130
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