Confocal fluorescence microscopy of living cells

Silvia M. Doglia; L. Bianchi; Roberto Colombo; N. Allam; Hamid Morjani; Michel Manfait; A. M. Villa

doi:10.1117/12.146189

17 June 1993 Confocal fluorescence microscopy of living cells

Silvia M. Doglia, L. Bianchi, Roberto Colombo, N. Allam, Hamid Morjani, Michel Manfait, A. M. Villa

Proceedings Volume 1922, Laser Study of Macroscopic Biosystems; (1993) https://doi.org/10.1117/12.146189
Event: Laser Spectroscopy of Biomolecules: 4th International Conference on Laser Applications in Life Sciences, 1992, Jyvaskyla, Finland

Abstract

We present here the study of intracellular distribution of doxorubicin in the single living cell by scanning confocal fluorescence microscopy, with argon laser excitation and photon counting detection. New results on the nuclear and cytoplasmic drug fluorescence not detectable by conventional microscopy are discussed. Differences in the fluorescence pattern observed in living and fixed cells suggest new insights in the mode of action of the drug.

Citation Download Citation

Silvia M. Doglia, L. Bianchi, Roberto Colombo, N. Allam, Hamid Morjani, Michel Manfait, and A. M. Villa "Confocal fluorescence microscopy of living cells", Proc. SPIE 1922, Laser Study of Macroscopic Biosystems, (17 June 1993); https://doi.org/10.1117/12.146189

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