17 June 1993 Confocal fluorescence microscopy of living cells
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Proceedings Volume 1922, Laser Study of Macroscopic Biosystems; (1993) https://doi.org/10.1117/12.146189
Event: Laser Spectroscopy of Biomolecules: 4th International Conference on Laser Applications in Life Sciences, 1992, Jyvaskyla, Finland
We present here the study of intracellular distribution of doxorubicin in the single living cell by scanning confocal fluorescence microscopy, with argon laser excitation and photon counting detection. New results on the nuclear and cytoplasmic drug fluorescence not detectable by conventional microscopy are discussed. Differences in the fluorescence pattern observed in living and fixed cells suggest new insights in the mode of action of the drug.
© (1993) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Silvia M. Doglia, L. Bianchi, Roberto Colombo, N. Allam, Hamid Morjani, Michel Manfait, and A. M. Villa "Confocal fluorescence microscopy of living cells", Proc. SPIE 1922, Laser Study of Macroscopic Biosystems, (17 June 1993); doi: 10.1117/12.146189; https://doi.org/10.1117/12.146189

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