17 June 1993 Effect of laser irradiation on the interaction of hematoporphyrin with physiological ions
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Proceedings Volume 1922, Laser Study of Macroscopic Biosystems; (1993) https://doi.org/10.1117/12.146194
Event: Laser Spectroscopy of Biomolecules: 4th International Conference on Laser Applications in Life Sciences, 1992, Jyvaskyla, Finland
Abstract
The effect of KCl ions on hematoporphyrin (HP) and its binding to DOPC liposomes were investigated. The absorption spectra of HP (2 - 30 micrometers ol/l) in KCl solutions (0.1 - 1.0 mol/l) showed two intensive peaks at 390 nm (monomer peak) and at 374 nm (dimer peak). When these samples were kept in the dark an agreement with HP aqueous spectra was observed. He-Ne laser (632 nm, 1 mW) illumination of the dye in KCl solution resulted in a change of the spectral appearance: both the original absorption bands became less distinguishable up to their disappearing and a new band at 404 nm was found. Its intensity was systematically enhanced as the time of illumination was increased (5 - 40 min.). This effect can be attributed to the laser energy mediated interaction of HP with ions.
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Libusa Sikurova, Libusa Sikurova, L. V. Zhorina, L. V. Zhorina, B. Cunderlikova, B. Cunderlikova, } "Effect of laser irradiation on the interaction of hematoporphyrin with physiological ions", Proc. SPIE 1922, Laser Study of Macroscopic Biosystems, (17 June 1993); doi: 10.1117/12.146194; https://doi.org/10.1117/12.146194
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