High-resolution correlation microscopy for monitoring of biological objects

Oleg V. Angelsky

doi:10.1117/12.146185

17 June 1993 High-resolution correlation microscopy for monitoring of biological objects

Oleg V. Angelsky

Proceedings Volume 1922, Laser Study of Macroscopic Biosystems; (1993) https://doi.org/10.1117/12.146185
Event: Laser Spectroscopy of Biomolecules: 4th International Conference on Laser Applications in Life Sciences, 1992, Jyvaskyla, Finland

Abstract

Two new optical methods for measuring size and velocity of quasi-spherical biological particles are proposed. One is an optical correlation method, based on the transformation of the transverse coherence function of the scattered field which forms the scattering particle images. A device for measuring the scattering particles size distribution function is proposed which has a response time of about 10 seconds, and can be applied to measure particles having sizes in the range of 3 to 500 micrometers to within 10%. The other is a holographic continuous exposure method for studying the dynamical and structure characteristics of the Brownian motion of unicellular algae. The measured algae velocity and size distribution functions are reported.

Citation Download Citation

Oleg V. Angelsky "High-resolution correlation microscopy for monitoring of biological objects", Proc. SPIE 1922, Laser Study of Macroscopic Biosystems, (17 June 1993); https://doi.org/10.1117/12.146185

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