Optical laser microspectroscopies within single living cells

Laurent Chinsky; M. Duquesne; A. Laigle; F. Sureau; Pierre-Yves Turpin; C. Amirand; Jean-Pierre Ballini; Paul Vigny

doi:10.1117/12.146187

17 June 1993 Optical laser microspectroscopies within single living cells

Laurent Chinsky, M. Duquesne, A. Laigle, F. Sureau, Pierre-Yves Turpin, C. Amirand, Jean-Pierre Ballini, Paul Vigny

Proceedings Volume 1922, Laser Study of Macroscopic Biosystems; (1993) https://doi.org/10.1117/12.146187
Event: Laser Spectroscopy of Biomolecules: 4th International Conference on Laser Applications in Life Sciences, 1992, Jyvaskyla, Finland

Abstract

Fluorescence and Raman spectroscopic analysis has been developed at the subcellular level, using laser excitation under the microscope. This approach has been successfully used on single living cells to monitor different biological molecular processes. The experimental applications which are presented here give an illustration of the performance of the method to obtain kinetic, topographic (spectral imaging), or quantitative data.

Citation Download Citation

Laurent Chinsky, M. Duquesne, A. Laigle, F. Sureau, Pierre-Yves Turpin, C. Amirand, Jean-Pierre Ballini, and Paul Vigny "Optical laser microspectroscopies within single living cells", Proc. SPIE 1922, Laser Study of Macroscopic Biosystems, (17 June 1993); https://doi.org/10.1117/12.146187

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