Vibrational Raman optical activity of biological molecules

L. D. Barron; Lutz Hecht; Z. Q. Wen; Steven J. Ford; A. F. Bell

doi:10.1117/12.146174

17 June 1993 Vibrational Raman optical activity of biological molecules

L. D. Barron, Lutz Hecht, Z. Q. Wen, Steven J. Ford, A. F. Bell

Proceedings Volume 1922, Laser Study of Macroscopic Biosystems; (1993) https://doi.org/10.1117/12.146174
Event: Laser Spectroscopy of Biomolecules: 4th International Conference on Laser Applications in Life Sciences, 1992, Jyvaskyla, Finland

Abstract

Advances in Raman optical activity (ROA) instrumentation based on the employment of a backscattering geometry together with a cooled backthinned CCD detector, a holographic notch filter, and a high-efficiency single-grating spectrograph have now enhanced the sensitivity to the level necessary to provide vibrational ROA spectra of most biological molecules in aqueous solution. Results on peptides and proteins show features originating in coupled C_(alpha )-H and N-H deformations of the peptide backbone which appear to be sensitive to the secondary conformation including loop and turn structures. Also carbohydrates show many features characteristic of the central aspects of carbohydrate architecture, with effects from the glycosidic link in oligosaccharides particularly prominent. Preliminary ROA spectra of pyrimidine nucleosides appear to reflect the mutual orientation of the sugar and base rings and the dominant furanose conformations.

Citation Download Citation

L. D. Barron, Lutz Hecht, Z. Q. Wen, Steven J. Ford, and A. F. Bell "Vibrational Raman optical activity of biological molecules", Proc. SPIE 1922, Laser Study of Macroscopic Biosystems, (17 June 1993); https://doi.org/10.1117/12.146174

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