In a previous paper, a 2D atomic force probe metrology system was described. The system consists of a 2D atomic force probe used in attractive mode (non-contact) with a laser inter- ferometer system for measuring sample displacement with nanometer sensitivity. The force probe consists of a square cantilever and specially designed 2D tip. The tip and cantilever are vibrated in 2D and the shift in resonance as the surface approaches is used for surface sensing. The system is able to measure submicrometer lines and trenches with accuracy and precision at the nanometer level. This paper will discuss operational characteristics of the system and the application of 2D AFM metrology to calibration of optical and scanning electron microscope system.